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. 2018 Jul 23;8:11089. doi: 10.1038/s41598-018-29432-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of tissue non-specific alkaline phosphatase increases pyrophosphate availability in human plasma. Post-hemodialysis plasma samples were incubated at 37 °C in the absence (−) or presence (+) of 100 µmol/L levamisole. (A) Quantification of plasma pyrophosphate levels (n = 8) at the indicated times. (B) Quantification of pyrophosphate levels in post-hemodialysis plasma samples (n = 40) after incubation for 24 hours. Results are presented as mean ± SEM, and were compared by the Wilcoxon matched pairs test. ***P < 0.001.