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. 2018 Jul 23;8:11089. doi: 10.1038/s41598-018-29432-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Loss of TNAP protein in post-dialysis plasma. (A) Total protein concentrations in plasma samples before and after dialysis. (B) Representative Coomassie Brilliant Blue staining of plasma proteins in two pairs of samples. (C) Representative immunoblot of TNAP levels in plasma samples before and after dialysis (upper), and relative level of TNAP level in post-dialysis relative to pre-dialysis samples (lower). (D) ELISA quantification of TNAP levels in plasma samples before and after dialysis. Results are presented as mean ± SEM of 40 pairs of samples, and were compared using the Wilcoxon matched pairs test. **P < 0.01; ***P < 0.001.