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. 2018 Jul 23;8:11089. doi: 10.1038/s41598-018-29432-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phosphate inhibits pyrophosphate hydrolysis. 32-Pyrophosphate (³²PPi) hydrolysis in rat aortic rings cultured in MEM (without phosphate) supplemented (+Pi) or not (−Pi) with 1 mmol/L phosphate. (A) Representative ³²PPi hydrolysis in the indicated time. (B) ³²PPi hydrolysis in absence or presence of 1 mmoL/L phosphate after 20 min of incubation. Results are presented as mean ± SEM of 12 rings (in three independent experiments), and were compared using the Wilcoxon matched pairs test. ***P < 0.001.