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. 2018 Jul 23;8:11067. doi: 10.1038/s41598-018-29330-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Silencing of OPN gene results in inhibition of cellular microcalcification formation in the knockdown clones. (A) Representative bright-field images showing alizarin red S stained cells for the four stably silenced clones and vector control. The scale bars represent 50 µm. (B) Bar plot showing mean and standard deviation of normalized alizarin red S stain intensity for stably silenced clones as percentage of vector control. Conventional Student t test threshold (p < 0.05) was considered statistically significant and is indicated by *.