Table 2.
The effect of rearing condition, blood meal vehicle, and dengue serotype on Ae. aegypti vector competence.
| Experiment | Rearing Condition | Bloodmeal vehicle | Serotype-strain | N | % (No.) infected | Mean virus titer (±1SEM) from infected bodies (log10pfu/body) | % (No.) Disseminated (total) | Mean virus titer ( ± 1SEM) from infected heads (log10pfu/head) | % Infections leading to dissemination |
|---|---|---|---|---|---|---|---|---|---|
| 1 | Bovine blood | Rabbit blood | DENV-2 NGC Proto | 62 | 16.1 (10) | 3.3 ± 0.3 | 14.5 (9) | 2.6 ± 0.3 | 90.0 |
| SkitoSnack | Rabbit blood | DENV-2 NGC Proto | 53 | 17.0 (9) | 3.4 ± 0.2 | 13.2 (7) | 2.6 ± 0.4 | 77.8 | |
| 2 | Bovine blood | Rabbit blood | DENV-2 NGC Proto | 18 | 38.9 (7) | 3.6 ± 0.3 | 38.9 (7) | 2.9 ± 0.3 | 100.0 |
| SkitoSnack | Rabbit blood | DENV-2 NGC Proto | 22 | 63.6 (14) | 3.3 ± 0.1 | 50.0 (11) | 2.6 ± 0.2 | 78.6 | |
| Bovine blood | SkitoSnack | DENV-2 NGC Proto | 12 | 25.0 (3) | 3.2 ± 0.2 | 25.0 (3) | 2.5 ± 0.2 | 100.0 | |
| SkitoSnack | SkitoSnack | DENV-2 NGC Proto | 8 | 25.0 (2) | 3.4 ± 0.05 | 25.0 (2) | 2.5 ± 0.2 | 100.0 | |
| Bovine blood | Rabbit blood | DENV-4 Dominica | 9 | 66.7 (6) | 2.7 ± 0.5 | 33.3 (3) | 2.2 ± 0.3 | 50.0 | |
| SkitoSnack | Rabbit blood | DENV-4 Dominica | 20 | 20.0 (4) | 2.6 ± 0.2 | 5.0 (1) | 1.4 | 25.0 |
Table shows the rearing condition, bloodmeal vehicle, dengue virus serotype, sample size, percent infected, mean virus titer of infected bodies, percent dissemination, mean virus titer of infected heads, and percent infections leading to dissemination data of 10th (experiment 1) or 12th generation (experiment 2) bovine blood-raised or SkitoSnack-raised Ae. aegypti females offered a dengue 2 virus-infected blood meal in rabbit blood or SkitoSnack and a dengue 4 virus-infected blood meal in rabbit blood.