Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 17;9:1664. doi: 10.3389/fimmu.2018.01664

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Westenius, Mäkelä, Julkunen and Österlund.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Infectivity of the H3N2, H5N1, and H7N9 viruses in the presence of trypsin. Monocyte-derived macrophages (Mɸs) (A) or monocyte-derived DCs (moDCs) (B) were infected with externally added TPCK-trypsin with A/Beijing/353/89 (H3N2), A/Vietnam/1194/2004 (H5N1), and A/Anhui/1/2013 (H7N9) influenza viruses with indicated multiplicity of infection (MOI) values and the samples were collected at 24 h after infection. (A) The proportion of infected Mɸs was analyzed with immunofluorescence microscopy by staining the cells with antibody against respective virus proteins. From each donor at least 300 cells (average ca. 550) were counted. (B) moDCs were stained with antibody against virus proteins and infected cells were analyzed with flow cytometry. From each donor 10,000 events were analyzed.