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. 2018 Jul 17;9:1664. doi: 10.3389/fimmu.2018.01664

Figure 8.

Figure 8

The productivity of H3N2, H5N1, and H7N9 infections in human innate immune cells. Monocyte-derived macrophages (Mɸs) or monocyte-derived DCs (moDCs) from four different donors were infected with A/Beijing/353/89 (H3N2), A/Vietnam/1194/2004 (H5N1), or A/Anhui/1/2013 (H7N9) influenza viruses at multiplicity of infection (MOI) of 0.01 and after 1 and 24 h of infection the supernatant samples were collected. (A,B) The infective viral titers produced from Mɸs (A) or moDCs (B) were determined by plaque assay in Madin–Darby Canine Kidney cells. The relative virus titers were calculated over 1 h samples. The horizontal lines represent the geometric means of the results from four blood donors. (C,D) The RNA was isolated from the supernatant samples from Mɸs (C) and moDCs (D) and the viral RNA levels were detected by qRT-PCR. The relative viral RNA expressions were calculated over 1 h samples with ΔCT method. The horizontal lines represent the geometric means from different donors. The statistical significance of differences between H5N1 and H3N2 or H7N9 was determined by Student’s t-test, *<0.05.