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. 2018 Jul 17;9:1569. doi: 10.3389/fmicb.2018.01569

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Copyright © 2018 Chen, Zhang, Chen, Cai, Zhang, Song and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CRISPRi efficiently represses the amb0994 gene transcription. (A) Western blot detection of dCas9 after SDS-PAGE with whole cell samples from experiment. The M represents the positions on the gel of the molecular mass markers (from top to bottom: 150, 100, and 70 kDa). (B) dCas9 transcription levels of the control (plasmid without sgRNA) and test strains in E. coli and M. magneticum AMB-1 cells (N = 3 per strain) were determined. The error bars represent the standard deviation of samples. (C) The relative expression levels of amb0994 in the KDamb0994 compared to control strains without sgRNA. The levels of transcription were calculated relative to the housekeeping gene rpoD (N = 9 per strain). The error bars represent the standard deviation of samples. *P < 0.05, **P < 0.01.