Skip to main content
. 2018 Jul 17;11:245. doi: 10.3389/fnmol.2018.00245

FIGURE 7.

FIGURE 7

Bioluminescence resonance energy transfer analysis of the effects of GHSR1a/OX1R heterodimerization on Gα protein subunit activation. HEK293 cells expressing GHSR1a, OX1R or BIFC (GHSR1a/OX1R) were stimulated with 100 nM ghrelin and/or 100 nM orexin-A for 20 min, and analysis of the interaction between GHSR1a/OX1R heterodimers and (A) Gαq-Rluc8, (B) Gαs-Rluc8, and (C) Gαi2-Rluc8 was performed using BRET. ∗∗p < 0.01, HEK293 cells co-expressing GHSR1a/OX1R stimulated with ghrelin (100 nM) vs. HEK293 cells expressing GHSR1a only;. The data points represent the mean ± SEM (N = 3) of four independent experiments. ns, not significant, as determined by two independent samples t-test.