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. 2018 Jul 17;9:1576. doi: 10.3389/fmicb.2018.01576

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Copyright © 2018 Deng, Deng, Yang, Li, Wang and Yuan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic tree of representative deduced bmoX sequences generated from different clone libraries of gas field soil samples (G) and reference sequences from GenBank. The tree was created using the neighbor-joining method with 1000 bootstrap replications; bootstrap values over 50% are shown at the branch nodes; the scale bars represents 10% sequence divergence. The GenBank accession number of all sequences used to build the tree is given in parentheses. The number of clones for each OPF is given in brackets [G-0/G-6/G-9/G-12/G-14]. Abbreviations for SDIMOs were as follows: Mmo, methane monooxygenase; PrmA, propane monooxygenase hydroxylase large subunit; pBmoX, putative butane monooxygenase alpha subunit; Mo, monooxygenase; pMo, putative monooxygenase. Sequences in clusters with pink background belong to group 3 SDIMOs; sequences in clusters with green background belong to group 5 SDIMOs; clusters with yellow background include both sequences belonging to group 3 and group 5 SDIMOs; clusters with gray background are unclassified SDIMOs and monooxygenases.