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. 2018 Jul 17;9:581. doi: 10.3389/fneur.2018.00581

Figure 5.

Figure 5

Synaptic changes at 3 and 28 days after intracerebral hemorrhage (ICH). Synapses in the ipsilateral striatum of mice after sham procedure (A), at 3 days after ICH (B), and at 28 days after ICH (C). Presynapses with transmitter vesicles are shown in blue; post-synapses are shown in yellow. The red asterisk in (B) indicates an autophagosome. (D) Quantification of synapse density at various time points after ICH. The number of synapses was normalized by 100-μm2 fields. Number of fields: sham, n = 71 fields; ICH day 3, n = 55 fields; ICH day 28, n = 23 fields. (E) Quantification of active area in synapses at various time points after ICH. Number of synapses: sham, n = 65; ICH day 3, n = 180; ICH day 28, n = 48. (F) Quantification of docked vesicles in synapses at various time points after ICH. Number of synapses: sham, n = 46; ICH day 3, n = 113; ICH day 28, n = 42. Results are shown as scatter graphs (mean ± SD). ***p < 0.001 vs. sham; ###p < 0.001 vs. ICH day 3; N.S., not significance. One-way ANOVA followed by Dunn's post-hoc analysis. Scale bars: (Ai,Bi,Ci) 500 nm; (Aii–iii,Bii–iii,Cii–iii) 100 nm. m: mitochondria. n = 6 animals per group. In (D), each dot represents one field; in (E,F), each dot represents one synapse.