FIGURE 1.

Flow chart describing the steps involved in CRISPR/Cas9 based genome editing. Step 1 describes the selection of gene and designing of gRNA, Step 2 describes the cloning of the gRNA in a suitable binary vector. Step 3 Shows the availability single and multiplex editing. Step 4 describes methods of transformation, Step 5 explains screening methods of edited crops and Step 6 demonstrates the evaluation and selection of the desirable transgene-free plant for the target trait.