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. 2018 Jul 17;9:985. doi: 10.3389/fpls.2018.00985

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Copyright © 2018 Jaganathan, Ramasamy, Sellamuthu, Jayabalan and Venkataraman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cas9 orthologs from bacterial species show differences in their PAM repertoire. (A) Sp-cas9 derived from Streptococcus pyogenes recognizes a three nucleotide PAM (5′-NGG) sequence. (B) Nme-cas9 derived from Neisseria meningitidis recognizes an eight nucleotide PAM (5′-NNNNGATT) sequence. (C) Sa-cas9 derived from Staphylococcus aureus recognizes a six nucleotide PAM (5′-NNGRRT) sequence. (D) St1-cas9 derived from Streptococcus thermophilus recognizes a seven nucleotide PAM (5′-NNAGAAW) sequence. (E) St2-cas9 derived from Streptococcus thermophilus recognizes a five nucleotide PAM (5′-NGGNG) sequence. Dotted lines indicate the site of the double-strand break.