Table 2.

List of CRISPR/Cas9 orthologs.

System	gRNA		Source	Protein	PAM (5′–3′)	Reference
CRISPR-cas9	tracrRNA+ crRNA		Streptococcus pyogenes	Cas9	NGG	Jinek et al., 2012
CRISPR-cpf1	Single RNA		Prevotella and Fracisella	Cas1, Cas2, Cas4	YTN	Zetsche et al., 2017
Ng-Ago	Single RNA		Natronobacterium gregoryi	Argonaute	Not required	Gao et al., 2016