Figure 4.
Comet speed decreases near the growth cone front.(A) Instantaneous speed of individual YFP-EB3 comets was grouped according to distance to growth cone front in wild-type and MAP1B-null growth cones. Error bars represent the median speed value and 95% confidence interval of comets located within a specific distance interval (e.g., below 1 μm, between 1 and 2 μm, etc.) from the growth cone front. Comets being closer than 2 μm to the growth cone front are slower than comets being further away. ***p < 0.001, Wilcoxon rank-sum test. (B) Quiver plots showing representative examples of comet movements in wild-type and MAP1B-null growth cones. Color and length of the individual arrows reflects instantaneous comet speed (displacement over 2 s). Scale bar, 5 μm.