Figure 5.

Differential probability distributions reveal growth cone area-independent, but selective influence of MAP1B over comet distance to growth cone front. Differential probability plots indicate detailed differences between distributions. Briefly, histograms with equal number, and size of bins were created from comet property values for each growth cone area group. Histogram values were divided by the total number of observations within each area bin, to obtain probability distributions. In order to monitor the effect of absence of MAP1B, probability distributions were subtracted from each other (WT minus MAP1B-null). Bars above 0 indicate a proportional increase within the specified intervals of wild-type growth cones, while bars below 0 show a proportional decrease (in wild-type growth cones as compared to MAP1B-null growth cones of the same area bins. Black lines indicate the bootstrapped median of differential probabilities, and red dashed lines indicate the 5 and 95 percentiles of the bootstrapped distributions. Note that distributions of EB3-comet distance to growth cone front display structured changes in with a switch point located ~ 2 μm from the growth cone front (marked by thick gray vertical lines), and that this specific switch point is found in each growth cone area bin. The analysis was based on at least 4,800 individual EB3-comet observations per growth cone area bin.