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. 2018 Jul 17;9:1622. doi: 10.3389/fimmu.2018.01622

Figure 1.

Figure 1

Manipulation of dietary vitamin D3 (VitD) results in robust changes in systemic VitD levels that are sex- and genotype dependent. Female and male B6 and PWD mice (N = 4 for each sex/strain combination) were assigned to VitD-high and VitD-low diets at 3 weeks of age. Serum samples were collected at the outset (Time = 0), and at 3 and 5 weeks post-treatment. 25(OH)D levels were measured by enzyme-linked immunoassay (see Materials and Methods). An overview of kinetic data is shown in (A), followed by comparisons at individual time points in (B–F), segregated by diet. The significance of the observed differences in (B–F) was assessed by two-way ANOVA, with Holm–Sidak’s post hoc comparisons: B6 vs. PWD (within sex), and female vs. male (within strain). Symbols indicate a significant difference between the indicated groups, as follows: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.