Table 1.
Illumina sequencing analysis of tissue Senegalese sole samples infected with nervous necrosis virus reassortants, and L-15-control group, at 48 h p.i.
| Sample | Total raw readsa | Total clean readsb | % contaminant sequences removed | Total unigenesc |
|---|---|---|---|---|
| Head kidney | ||||
| L-15 | 331,555,320 | 321,395,622 | 3.1 | – |
| wSs160.03 | 269,346,896 | 263,149,296 | 2.3 | 28,954 |
| rSs160.03247+270 | 303,553,076 | 296,703,761 | 2.3 | 28,964 |
| Eye/brain | ||||
| L-15 | 289,923,568 | 231,003,910 | 20.3 | – |
| wSs160.03 | 310,151,374 | 245,611,206 | 20.8 | 27,135 |
| rSs160.03247+270 | 293,997,192 | 238,197,453 | 19 | 27,491 |
aTotal number of read-pairs (or reads for single-end runs) that passed Illumina filter.
bTotal number of clean reads after the analysis with the SeqTrimNext pipeline.
cTotal number of unigenes after mapping with bowtie2 using Solea DB (v.4.1 2014-01-23) as reference transcriptome.