Bioluminescence images of luciferase knockdown in vivo after injection of luciferase siRNA containing FRα targeted micelleplexes (A–D) and scramble siRNA containing FRα targeted micelleplexes (E–H). Time points displayed here are 0 hours (A, E), 24 hours (B, F), 48 hours (C, G), and 72 hours (D, H). Luciferase activity is shown predominantly in the primary tumor as well as in metastases.