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. 2018 Jul 17;9:1033. doi: 10.3389/fpls.2018.01033

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Copyright © 2018 Xu, Liu, Zhu and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Functional analysis of the ZmNBS25 gene in rice. (A) Chromatogram of SA extracted from ZmNBS25-OE and control (EV) rice plants without pathogen inoculation. STD represents standard SA; mAU indicates peak height. The arrow indicates the SA peak. (B) Resistance of ZmNBS25-OE plants to rice sheath blight. (C) Expression levels of defense related genes in ZmNBS25-OE and control plants inoculated with Ralstonia solani. Data were normalized using the transcript level of Ostubulin and 18srRNA. OE5 and OE7 represent two different transgenic lines of ZmNBS25-overexpressed rice. The expression levels of genes in R. solani-infected EV plants were used as controls and assigned the value of 1. Asterisks indicate statistically significant differences between treated and untreated maize by Student’s t-test (^∗P< 0.05, ^∗∗P< 0.01, ^∗∗∗P< 0.001). Data represent mean relative expression values ± standard deviation from three independent experiments.