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. 2018 Jul 17;9:1592. doi: 10.3389/fmicb.2018.01592

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Copyright © 2018 Szafran, Kołodziej, Skut, Medapi, Domagała, Trojanowski, Zakrzewska-Czerwińska, Sriram and Jakimowicz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The influence of MsTopA inhibition on the number of DnaN-EGFP complexes. (A) Canonical cell cycle of M. smegmatis visualized by TLMM. Phase C corresponds to chromosome replication. It starts with the appearance of a single DnaN-EGFP focus (time 0) (green dot), which subsequently splits into two foci. Phase C terminates after 130 min with the dismantling of the replisome complex. Phase D+B (30 min) is the time between the termination of DNA replication and cell division and the time after cell division prior to initiation of replication. Scale bar, 5 μm (B) TLMM analysis of M. smegmatis treated with 120 μM amsacrine or 30 μM XC-4. Analyzed cells to be followed are marked with white arrows. Scale bar, 5 μm (C) The percentage of cells with a different number of DnaN-EGFP foci (none, one, two or more than two) in M. smegmatis cultures treated with different concentrations (30–240 μM) of amsacrine or its derivatives (XC-1, XC-2, XC-3, and XC-4). The number of cells used for statistics varies from 300 to 400. (D) The influence of increasing concentration of amsacrine and XC-4 on the number of DnaN-EGFP complexes.