Table 1.
Strains used in this study.
| Strain | Relevant genotype or description | Source |
|---|---|---|
| E. coli | ||
| DH5α | supE44ΔlacU169(φ80 lacZΔM15)hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Lab stock |
| BL21 | B F−dcm ompT hsdS(rB−mB−) gal | Lab stock |
| S. venezuelae | ||
| ATTC10712 | Wild-type/laboratory strain | Lab stock |
| M. smegmatis | ||
| mc2155 | Laboratory strain | Lab stock |
| JH01 | M. smegmatis mc2155 enzyme or DNA rathednaN-egfp | Trojanowski et al., 2015 |
| MSZ1 | M. smegmatis mc2155 topA::apra pMV306-MstopA | This study |
| MSZ2 | M. smegmatis mc2155 topA::apra pMV306-MttopA | This study |
| PS17 | M. smegmatis mc2 ΔtopA, dnaN-egfp, parB-mcherry, attBL5::pTC-28S15-0X psmyctetRrevpmyc1topA | This study |
| PS29 | M. smegmatis mc2, dnaN-egfp, parB-mcherry, attBL5::pTC-28S15-0X psmyctetRrevpmyc1 | This study |