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Mouse lethality assay. Ten mice were infected intraperitoneally with 104 CFU each of the WT, ΔfrwC, and C-frwC strains, and the corresponding survival curves for 14 days were calculated. The survival percentages showed significant differences between the WT and mutant strains (P < 0.001; Kaplan-Meier test), but the WT and complemented strains exhibited no difference (P = 0.114; Kaplan-Meier test).
