FIG 9.

TPL2 knockout reduces MAPK activation. (A) BMDCs from TPL2-floxed (Flox) or TPL2 knockout (TPL2-KO) mice were exposed to TcdB (200 ng/ml) in 24-well plates for 30 min. (B) Cecal tissues from Flox or TPL2-KO mice infected with C. difficile were harvested, lysed, and used for determination of the activation of TPL2, ERK, p38, and JNK by Western blot analysis. Experiments were repeated 3 times, and representative results are shown. Asterisks indicate significant differences (*, P < 0.01) from results for the control.