FIG 7.

Immunoblot probed with peroxidase-conjugated anti-human immunoglobulin IgA showing IgA protease expression of sequential isolates of a persistent NTHi strain from patient 56 over the course of 217 days. Visit number, the monthly clinic visit; IgA expression, the IgA protease variant expressed; TCAAAAT repeats, the number of repeats in the igaB2 gene; Frame, whether the number of repeats places the igaB2 gene in frame or out of frame. Molecular mass markers (in kilodaltons) are shown on the left. The lower blot shows IgA protease expression from cultures grown from individual colonies obtained from the original culture of sputum collected at visit 34.