FIG 5.

TLR2 activation by triacylated synthetic peptides (P3C) caused F-actin formation. A 384-well black microtiter plate was seeded with HEK-0 cells (A) or HEK-TLR2 cells (B) for 24 h prior to incubation with 10 ng of IL-8 or P3C for 2 h. F-actin was stained with ActinGreen 488 ReadyProbes reagent (Thermo Fisher). The amount of F-actin formation in treated cells was determined by measurement at 495 nm for excitation and 518 nm for emission, with measurements normalized to those for the untreated control (C). The experiments we carried out in triplicate in two independent replications. Statistical significance was calculated by using Student's t test (*, P < 0.05; ns, no significance).