FIG 5.

SchuS4 induces the rapid loss of ATP and oxygen consumption and an increase in mTOR activity in BMM, indicative of oncosis. (A) ATP levels in BMM at 4, 8, 24, and 48 h following SchuS4 infection (MOI, 5, 50). (B) LDH assay on mock- and SchuS4-infected macrophages (MOI, 5 and 50) at 24 and 48 h. (C) Real-time trace of basal OCR in mock-infected versus SchuS4-infected macrophages (MOI, 50). (D and E) Representative Western blots (D) and corresponding densitometry analysis (E) of phosphorylated S6K (S6-P) levels in SchuS4-infected BMM (MOI, 5, 50, and 500 at 24 h). Cells were pretreated with Torin-1 (500 nM) or the vehicle as a control. Western blot images and densitometry, OCR traces, and confocal images with the corresponding TMRE fluorescent intensity analysis results are representative of those from 3 separate experiments. Otherwise, data are presented as the mean ± SEM (3 technical replicates) from one representative experiment of three repeats with similar results. P values were determined using Student's t test. *, P < 0.05; **, P < 0.01; ****, P < 0.0001.