FIG 5.

Primary human neutrophils and monocytes are induced by PIP₃ to phagocytose nonmotile P. aeruginosa. Primary human neutrophils from healthy donors (A and D), primary human neutrophils from CF patients (B and E), and primary human monocytes from healthy donors (C) were assayed for relative phagocytosis of PA14 motAB motCD and PA14 waaL motAB motCD, as indicated, without or after, treatment of cells with 12 μM PIP₃. The data were normalized to phagocytosis of bacterial strains without PIP₃ treatment and are represented as fold increase in phagocytosis. An MOI of 10 was used for all experiments. All data were analyzed using an unpaired t test with Welch's correction and are representative of at least two independent biological experiments (for A, C, D, E, n ≥8; for B, n ≥4). ***, P ≤ 0.0005; **, P ≤ 0.005, for results compared to those in the absence of PIP₃ treatment.