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OAg changes are driven by intracellular conditions. (A) Electrophoresis profile of LPS in LB and N-minimal media. E. coli strain O55:B5 (lane 1) and the S. Typhimurium WT (lanes 2 and 3), ΔvisP (lanes 4 and 5), and complemented visP+ (lanes 6 and 7) strains were used. (B) qRT-PCR analysis of wzy polymerase in LB and N-minimal media. Relative fold expression in the WT, ΔvisP, ΔwzzfepE, ΔwzzST, ΔvisP wzzfepE, and ΔvisP wzzST strains is shown. **, P < 0.01; ****, P < 0.0001.
