FIG 2.

Roles of OAg and VisP in S. Typhimurium pathogenesis. (A) qRT-PCR analysis of genes involved in the OAg biosynthesis pathway. relative fold expression of the pmrB, wzz_fepE, wzz_ST, and phoP genes in the WT, ΔvisP, and complemented visP⁺ strains grown in LB is shown. (B to D) J774 phagocytosis (B), internalization (C), and long-period replication (D) of the WT, ΔvisP, and complemented visP⁺ strains. (E and F) qRT-PCR analysis of the sifA (N-minimal growth condition) (E) and mgtB (LB growth condition) (F) genes. Relative fold expression in the WT, ΔvisP, and complemented visP⁺ strains is shown. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.