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. 2018 Jul 23;86(8):e00319-18. doi: 10.1128/IAI.00319-18

FIG 3.

FIG 3

The interplay of VisP and PCP determines the final length of OAg. (A) qRT-PCR analysis of wzz expression in LB and N-minimal media. Relative fold expression in the WT, ΔvisP wzzfepE, and ΔvisP wzzST strains is shown. (B) High-resolution electrophoresis (15% SDS-PAGE) and staining with a ProQ Emerald 300 LPS gel staining kit (Thermo Fisher, Waltham, MA, USA) of the LPS profiles of the ΔvisP wzzfepE (lanes 1 and 2) and ΔvisP wzzST (lanes 3 and 4) strains grown in LB and N-minimal media. (C) Gradient SDS-PAGE (4 to 12%) of the LPS profile stained with a ProQ Emerald 300 LPS gel staining kit (Thermo Fisher, Waltham, MA, USA), showing the core lipid A and different OAg chain lengths in the WT, ΔwzzfepE, and ΔwzzST strains, all grown in LB. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.