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Changes in flagellar gene expression with different OAgs. (A) qRT-PCR analysis of genes involved in flagellar assembly and motility. relative fold expression of the fliA, flhDC, and motA genes in the WT, ΔvisP, and complemented visP+ strains grown in LB is shown. (B) qRT-PCR of the flagellin gene fliC, swimming motility assay, and immunoblotting performed with anti-FliC and anti-RpoA (control) monoclonal antibodies. ***, P < 0.001; ****, P < 0.0001.
