Fig. 3.

Snail-overexpressed fibroblasts-conditioned media (CM) induce 5-fluorouracil (5-FU)/paclitaxel (PTX) resistance in colorectal cancer cells in vitro. (A) Murine colorectal cancer CT26 cells were treated with the indicated dose of 5-FU/PTX alone or co-cultured with 30%/60% 3T3-mock/3T3-Snail-conditioned media for 48 hours, and the cell viability was examined by Cell Counting Kit 8 assay. The IC50 values of 5-FU/PTX for CT26 were determined (*p < 0.05). (B) The levels of the poly(ADP-ribose) polymerase (PARP), cleaved PARP, caspase-3, and cleaved caspase-3 proteins from CT26 alone or co-cultured with 3T3-mock/3T3-Snail CM were analyzed by Western blotting. 3T3-Snail CM contained something induced 5-FU/PTX resistance in CT26.