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. 2018 Jul 24;18:46. doi: 10.1186/s12896-018-0457-7

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Quenching of NicA2 activity in blood in vitro by MeOH. MeOH was added in vitro to blood containing 40 ng/ml nicotine before or after adding NicA2. Prior addition of MeOH completely quenched NicA2 activity (no difference from BSA control). In contrast, adding MeOH after an approximately 10 s exposure of nicotine to NicA2 in vitro allowed degradation of nicotine to below the limit of assay detection. *** p < 0.001 using two-tailed unpaired t tests with Welch’s correction. Mean ± SD, n = 8/group