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. 2018 Jul 23;9:18. doi: 10.1186/s13227-018-0107-0

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — The developing central nervous system of Molgula occidentalis. a Dorsal view of a neurula stage Molgula occidentalis embryo. Green shows fluorescent in situ hybridization for Celf3/4/5 (also known as Etr-1) transcription. b In situ hybridization for Neurogenin in initial tailbud embryo, showing transcription in the embryonic brain, motor ganglion (MG), and bipolar tail neurons (BTNs). c In situ hybridization for Onecut, expressed in brain and MG. d Two-color fluorescent in situ hybridization showing co-expression of Ebf (magenta) and Slc18a3 (also known as VAChT, green) in the MG. Embryo outlined by dotted lines. Anterior is to the left in all images. All scale bars = 25 µm