Table 2.
Examples of HS-dependent changes in non-coding RNAs
| Gene ID | Description | WT Norm Avg. | WT Heat Avg. | log2- CHG | Sig. adj. p-val. | Mut Norm Avg. | Mut Heat Avg. | log2-CHG | Sig. adj. p-val. | Ratio of pollen to seedlinga |
|---|---|---|---|---|---|---|---|---|---|---|
| Micro RNAs, 6 total for WT, 4 shared by cngc16 | ||||||||||
| AT2G25095 | MIR156A | 13.30 | 112.42 | 2.84 | Y | 18.26 | 80.45 | 1.99 | Y | 4 |
| AT4G31877 | MIR156C | 2.64 | 17.79 | 2.17 | Y | 3.54 | 14.85 | 1.62 | Y | 1 |
| AT2G39175 | MIR160A | 1.89 | 15.10 | 2.17 | Y | 7.75 | 12.67 | 0.62 | N | 3 |
| AT2G25011 | MIR836A | 4.16 | 23.65 | 2.06 | Y | 7.03 | 18.07 | 1.18 | N | 330 |
| AT2G24103 | MIR831A | 21.97 | 8.70 | −1.21 | Y | 48.24 | 9.15 | −2.23 | Y | 144 |
| AT4G14811 | MIR780A | 131.10 | 47.38 | −1.41 | Y | 219.82 | 66.31 | −1.68 | Y | 52,756 |
| Anti-Sense Long RNAs, 9 total for WT, 7 shared by cngc16 | ||||||||||
| AT1G04533 | overlaps AT1G06923 | 0.37 | 7.29 | 2.11 | Y | 0.00 | 4.52 | 1.68 | N | NA |
| AT2G09430 | overlaps AT2G43235 | 303.78 | 87.30 | −1.72 | Y | 329.94 | 57.35 | −2.41 | Y | NA |
| Long Non-Coding, 40 total for WT, 31 shared by cngc16 | ||||||||||
| AT5G04635 | NA | 12.13 | 317.30 | 4.36 | Y | 10.99 | 220.83 | 3.94 | Y | NA |
| AT5G04815 | NA | 55.99 | 8.40 | −2.50 | Y | 57.14 | 10.29 | −2.29 | Y | NA |
| Small Nucleoar and Nuclear RNAs, 10 total for WT, 7 shared by cngc16 | ||||||||||
| AT4G04475 | NA | 0.00 | 6.28 | 2.24 | Y | 0.00 | 3.33 | 1.43 | N | NA |
| AT3G57645 | U2.2 | 15.21 | 1.26 | −2.45 | Y | 38.85 | 5.27 | −2.44 | Y | 20 |
| Other RNAs, 14 total for WT, 10 shared by cngc16 | ||||||||||
| AT5G28824 | NA | 0.00 | 21.53 | 3.50 | Y | 0.00 | 11.77 | 2.57 | Y | 17 |
| AT1G64563 | NA | 1573.5 | 300.01 | −2.33 | Y | 1130.83 | 187.96 | −2.52 | Y | 15 |
aRatio of expression between pollen and seedling is based on [22]
Norm Normal, Avg Average, log2-CHG log2-fold change, Sig. adj. p-val: significance based on adjusted p-value ≤0.01, Mut: cngc16, N No, Y Yes