Table 3.
HS-dependent changes in WT that were most different in cngc16 under HS
| Gene ID | Description | Net-work a | Function based on S (String), MM (MapMan), T (TAIR) | WT Heat Avg. | cngc16 Heat Avg. | log2- CHG | Sig. adj. p-val. | Seed-ling HS log2- CHGb | Ratio pollen to seed-ling c |
|---|---|---|---|---|---|---|---|---|---|
| Over-responsive in cngc16 compared to WT under HS | |||||||||
| AT4G33790 | FAR3, CER4, acyl CoA reductase | B | Cell Wall, Cuticular Wax S | 5.6 | 30.4 | 2.1 | 0.001 | 0.16 | 0.03 |
| AT3G05650 | Receptor-like protein 32, RLP32 | Biotic Stress Signaling S | 20.4 | 73.2 | 1.7 | 0.009 | −0.58 | < 0.01 | |
| AT3G44310 | Nitrilase 1 | Secondary Metabolism MM | 54.1 | 146 | 1.4 | < 0.001 | −0.43 | < 0.01 | |
| AT5G59320 | Lipid transfer protein 3 | C | Membrane Dynamics S | 131 | 366 | 1.4 | 0.004 | 0.32 | 0.06 |
| AT5G25280 | Serine-rich protein | Unknown S,MM, T | 146 | 376 | 1.3 | 0.010 | 2.05 | 0.01 | |
| AT5G59613 | ATP synthase | ATP Synthase T | 31.5 | 78.3 | 1.2 | 0.001 | NA | 0.08 | |
| AT5G15960 | Stress-responsive protein (KIN1) | Stress Protein, Antifreeze T | 58.4 | 139 | 1.2 | 0.005 | 0.09 | 0.00 | |
| AT5G59310 | Lipid transfer protein 4 | C | Membrane Dynamics S | 377 | 879 | 1.2 | 0.004 | 0.32 | 0.17 |
| AT2G24940 | MAPR2 steroid-binding protein 3 | Membrane Dynamics S | 40.9 | 91.8 | 1.1 | 0.009 | 0.15 | 0.02 | |
| AT3G04120 | GAP dehydrogenase C subunit 1 | B | Glycolysis MM | 160 | 338 | 1.0 | 0.001 | 0.12 | 0.01 |
| AT1G07590 | Tetratricopeptide repeat (TPR)-like | Gene Expression MM | 66.5 | 142 | 1.0 | 0.006 | 0.15 | 0.02 | |
| Under-responsive in cngc16 compared to WT under HS | |||||||||
| AT2G20110 | Tesmin/TSO1-like with CXC domain | Gene Expression MM | 120 | 51.6 | −1.2 | 0.002 | 0.20 | 2.60 | |
| AT1G49490 | LRX9, Leucine-Rich Repeat/Extensin 9 | Cell Wall Functions MM | 149137 | 61672 | −1.2 | < 0.001 | 0.09 | 702 | |
| AT2G46192 | Non-coding RNA, “other_rna” | Gene Expression MM | 91.0 | 33.6 | −1.4 | < 0.001 | NA | 2.57 | |
| AT5G17320 | HDG9, homeodomain GLABROUS 9 | Gene Expression MM | 37.0 | 11.6 | −1.5 | 0.010 | 0.24 | 120 | |
| AT4G12870 | Lysosomal thiol (GILT) reductase | A | Unknown S,MM, T | 20.3 | 5.8 | −1.6 | 0.007 | NA | 0.02 |
| AT3G08770 | Lipid transfer protein 6 | A | Membrane Dynamics S | 108 | 31.2 | −1.6 | < 0.001 | −0.53 | < 0.01 |
| AT1G56100 | Invertase/pectin methylesterase inhibitor | A | Cell Wall Functions MM | 21.7 | 3.2 | −2.3 | < 0.001 | 0.02 | < 0.01 |
| AT2G46960 | CYP709B1, cytochrome P450 | A | Stress Responsive P450 S | 17.8 | 2.3 | −2.3 | 0.002 | 0.29 | 6.63 |
| AT5G47350 | Alpha/beta-Hydrolases superfamily | A | Membrane Dynamics T | 43.8 | 6.0 | −2.5 | < 0.001 | 0.07 | < 0.01 |
| AT3G20210 | Delta vacuolar processing enzyme | A | Protein Degradation S | 31.0 | 3.7 | −2.6 | < 0.001 | 0.07 | 2644 |
| AT4G15750 | Invertase/pectin methylesterase inhibitor | A | Cell Wall Functions MM | 51.7 | 5.2 | −2.9 | < 0.001 | 0.22 | 17.1 |
| AT1G26240 | Proline-rich extensin-like family | Cell Wall Functions MM | 14.4 | 0.4 | −3.2 | < 0.001 | 0.09 | 0.50 | |
aNetwork group analysis was done using STRING [32]
bHS-dependent changes in transcript abundance in seedlings (aerial parts) based on publically available data using the AtGenExpress Visualization Tool (AVT) (http://jsp.weigelworld.org/expviz/expviz.jsp) [33] for seedlings exposed to one hour HS at 38 °C. The log2-fold change was calculated based on a comparison of means of normalized values for two heat-stressed and two non-stressed seedling samples
cRatio of expression between pollen and seedling is based on [22]. NA stands for not applicable because it was not possible to calculate based on the information available
Functional annotation based on S (STRING, [32]), MM (MapMan, [71]), and T (TAIR, [64]), as noted