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. 2018 Jul 17;12:51. doi: 10.3389/fnana.2018.00051

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Copyright © 2018 Simhal, Gong, Trimmer, Weinberg, Smith, Sapiro and Micheva.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pipeline of the Synaptic Antibody Characterization Tool (SACT). SACT combines a probabilistic punctum detector (top row) with a probabilistic synapse detector (Simhal et al., 2017) to determine the properties of the candidate antibody.