Table 2.
Results from pairwise antibody comparisons.
| Target protein | Candidate antibodies | Reference antibody | Punctum density (μm3) | Average punctum size / standard deviation (pixels) | Target synapse density (μm3) | Target specificity ratio |
|---|---|---|---|---|---|---|
| Synapsin | Synapsin GP | PSD-95 | 2.01 | 15.9 / 59.4 | 0.55 | 0.275 |
| Synapsin R | 1.09 | 27.0 / 48.6 | 0.5 | 0.457 | ||
| VGluT1 | VGluT1GP | Synapsin | 1.54 | 12.1 / 30.2 | 0.44 | 0.283 |
| VGluT1M | 1.27 | 11.0 / 22.4 | 0.33 | 0.257 | ||
| PSD-95 | PSD-95M | Synapsin | 0.95 | 10.7 / 101.7 | 0.54 | 0.568 |
| PSD-95R | 1.14 | 25.4 / 39.9 | 0.79 | 0.691 | ||
| Gephyrin | GephyrinL106 | GAD | 0.72 | 12.5 / 94.1 | 0.13 | 0.181 |
| GephyrinBD | 0.46 | 17.9 / 263.1 | 0.08 | 0.175 | ||
| Cav 3.1 | Cav3.1M | VGluT1 | 0.58 | 7.4 / 14.0 | 0.33 | 0.568 |
| Cav3.1R | 1.22 | 8.6 / 52.3 | 0.33 | 0.271 |
Punctum density, average punctum size, and standard deviation of punctum size were all computed for each antibody tested. The names in bold in the second column represent the antibodies preferred by an expert based on visual examination. In each case, the measures automatically computed by the framework agree with the expert observer's judgement.