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. Author manuscript; available in PMC: 2018 Jul 24.
Published in final edited form as: Nat Protoc. 2017 Jul 27;12(8):1683–1701. doi: 10.1038/nprot.2017.054

Figure 1.

Figure 1

Overview of multiplexed workflow for discovery proteomics in plasma. This figure shows application of the workflow to a specific study in which iTRAQ 4-plex reagent was used to process and analyze four time points of planned myocardial infarction (PMI) patient samples. However, as shown in the protocol, other depletion and labeling strategies can be used to accommodate the goals of a given study. HCD, higher energy collisional dissociation. Image adapted with permission from Keshishian et al.38, American Society for Biochemistry and Molecular Biology.