. Author manuscript; available in PMC: 2018 Jul 24.

Published in final edited form as: Nat Protoc. 2017 Jul 27;12(8):1683–1701. doi: 10.1038/nprot.2017.054

TABLE 1.

Plasma volume requirement for labeling after IgY14-only and IgY14–SuperMix depletions.

Protein amount for labeling post-depletion (μg)	IgY14 only		IgY14-SuperMix
Protein amount for labeling post-depletion (μg)	Min. nondepleted plasma volume required (μL)^a	Min. plasma volume to deplete (μL)^b	Min. nondepleted plasma volume required (μL)^a	Min. plasma volume to deplete (μL)^b
10	4.1	6.1	28.6	42.9
30	12.2	18.4	85.7	128.6
50	20.4	30.6	142.9	214.3
100	40.8	61.2	285.7	428.6

Volume requirement is based on the 3.5 and 0.5% minimum recovery after IgY14-only and IgY14–SuperMix depletions, respectively. A plasma concentration of 70 mg/ml was used for the calculations.

Minimum required volume is increased by 50% to account for losses during sample processing.