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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: J Struct Biol. 2018 Mar 14;203(2):81–89. doi: 10.1016/j.jsb.2018.03.005

Figure 6. Co-immuniprecipitation assays.

Figure 6.

(A) The ability of V5-BcSEFIR to co-immunoprecipitate with the HA-mutations or wild type HA-BcSEFIR was shown. Mutagenesis on asymmetric interface markedly reduced BcSEFIR self-association. (B) The ability of V5-BcSEFIRΔtail to co-immunoprecipitate with the HA-mutations or wild type HA-BcSEFIRΔtail was shown. Mutagenesis on symmetric interface markedly reduced BcSEFIRΔtail self-association. WT, wild type recombinant protein. Asym, mutant with key interacting amino acids on asymmetric binding site substituted by Alanine. Sym, mutant with key interacting amino acids on symmetric binding site substituted by Alanine.