Figure 3.

Treatment with PYR-41 or thalidomide has not increased IL-12 secretion. Murine bone marrow-derived DC (cultured for 4 d) conferred PYR-41 (5 μM), thalidomide (30 μM), LPS (10 ng/ml), or DMSO stimulation for 12 hrs, and IL-12 expression was determined by flow cytometry with intracellular IL-12 staining. The positive percentages (a) and the mean fluorescence intensity (b) of analyzed population were shown. Data were presented as the mean ± SEM and one-way ANOVA with Newman–Keuls post test. One representative from 3 independent experiments was shown.