Figure 1.

Nucleotide sequence of RPC10 coding and flanking regions. The uninterrupted open reading frame of 210 bp encodes a sequence of 70 amino acids which contains the two sequenced tryptic peptides (underlined). The four cysteines of the putative zinc binding domain are circled. The basic heptapeptide discussed in the text is underlined (dotted line). In the upstream region, a consensus ABF1 binding site and a T-rich element are boxed. The position of the Nru I and EcoN I restriction sites used for gene disruption are indicated.