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. 2018 Jul 24;2(4):391–407.

Figure 6.

Figure 6

Ability of Tat to facilitate transcription preinitiation complex formation in the absence of nascent RNA synthesis. A. Effect of preincubation of HIV template and Tat in the presence of various nucleoside triphosphates. Different combinations of nucleoside triphosphates were added to in vitro transcription reactions containing the HIV CD12 promoter template (75 ng) without (lanes 1–3) or with (lanes 4–6) 0.4 μM Tat. After a 30-minute preincubation, 0.05% sarkosyl and then a full complement of nucleoside triphosphates were added to the reactions. B. Effect of limiting exogenous nucleoside triphosphates on HIV-1 transcription in vitro. Different combinations of nucleoside triphosphates were added to in vitro transcription reactions containing 300 ng of either AdMLP or HIV-1 LTR DNA templates. Transcription reactions were analyzed after a 30-minute incubation.