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. 2018 Jul 24;2(3):285–296.

Figure 3.

Figure 3

Effect of the removal of the SV40 intron and splicing sites on the ability of the SV40 promoter to generate PCNA mRNA levels. We used the construct in which the SV40 intron and splicing sites were removed from the promoter (see Materials and Methods) to cotransfect TK−tsl3 cells together with pTK 11. Total RNA was extracted and analyzed as described in Figure 2. Lane 1, quiescent cells; lane 2, serum-stimulated cells at the permissive temperature; lane 3, serum-stimulated cells at the restrictive temperature.