Figure 11.
Spl and PRF-1 are distinct nuclear factors. The HSA −67/−38 probe was incubated with purified Spl (lanes 1–6) and PMB nuclear extract (lanes 7–12). Lanes 1, 6, 7, and 12 were control electrophorec tic mobility shift assays that did not contain any competitor DNAs. Lanes 2–5 and 8–11 contained 10-, 20-, 40-, and 80-fold molar excess of an Spl consensus sequence.