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. 2018 Jul 24;2(3):241–257.

Figure 9.

Figure 9

Transient CAT assays in C2C12 cells demonstrating the effect of the Id (inhibitor of differentiation) helix-loop-helix protein on deleted α-actin promoters. Lanes 1–8 of each assay contained the following plasmids: A, pHSA87CAT-3′SV M1; B, pHSA87CAT-3′SV M1,M5; C, pHSA37/30CAT-3′SV; and D, pHSA37/30CAT-3′SV M5 (6 μg). All plasmids included the full-length native human skeletal α-actin promoter. Lanes 3–5 also contained 0.5, 1, and 2 μg of co-transfected pEMSV-E12(A.S.). Lanes 6–8 also contained 0.5, 1, and 2 μg of co-transfected pEMSV-Id. The total amount of DNA in each transfection was kept at a constant 10 μg with the carrier plasmid pUC18.