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. 2018 Jul 24;2(2):147–160.

Figure 2.

Figure 2

Southern analysis of the pMT-993S-transfected cell lines. Genomic DNA isolated from wild-type F9 cells (F9-wt) and from the transfected cell lines F9-N-4, F9-Hl.6-5, and F9-H1.6-10 was digested with BamH I and analyzed by Southern blot. The DNA on the Southern blot was hybridized to randomly primed probe to the translated portion of the Hox 1.6 cDNA. The 1.9 Kb fragment corresponds to the endogenous Hox 1.6 gene, and the 1.2 Kb fragment corresponds to the integrated Hox 1.6-993 cDNA insert. Exposure time was 24 hours at −70°C with an intensifying screen.