Figure 5.

Western analysis of the Hox 1.6 protein. Western analysis of the Hox 1.6 protein in control and pMT-993S-transfected cells is shown. Cells were grown as described in Figure 2 and harvested after 0, 24, or 72 hours of treatment, as indicated. 600 μg of total cell protein were resolved by SDS-PAGE, transferred to nitrocellulose, probed with a polyclonal antisera to Hox 1.6 (see Materials and Methods), and visualized using ¹²⁵I-labeled protein A; exposure time was 21 days. This experiment was performed two times. Qualitatively similar results were observed in both experiments for all extracts except from F9-H 1.6-5 cells treated with RACT for 72 hours; a second experiment detected amounts comparable to those observed in control F9-N-4 cells treated with RACT for 72 hours (data not shown).